Coding

Part:BBa_K4348031:Design

Designed by: Rylan Donohoe   Group: iGEM22_McGill   (2022-09-17)


AKR1D1_V121toL_his


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 753
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 753
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 753
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 753
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 753
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Residue was mutated through PCR mutagenesis


Source

Homo sapiens

References